Part:BBa_J176127

V0120

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

V0120, standard high-copy cloning vector for E. coli

Usage and Biology

This high-copy vector carries RFC23 (Silver Standard) BioBrick cloning sites for modular protein assembly.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 101
  Illegal XbaI site found at 116
  Illegal SpeI site found at 1825
  Illegal PstI site found at 1839
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 101
  Illegal SpeI site found at 1825
  Illegal PstI site found at 1839
  Illegal NotI site found at 107
  Illegal NotI site found at 247
  Illegal NotI site found at 1832
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 101
  Illegal BglII site found at 1015
  Illegal BamHI site found at 318
  Illegal BamHI site found at 1021
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Illegal suffix found at 1825
  Illegal EcoRI site found at 101
  Illegal XbaI site found at 116
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 101
  Illegal XbaI site found at 116
  Illegal SpeI site found at 1825
  Illegal PstI site found at 1839
  Illegal NgoMIV site found at 4648
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 1571
  Illegal BsaI.rc site found at 3216
  Illegal SapI site found at 2133